Journal: PLoS Pathogens
Article Title: Human low-density lipoprotein receptor plays an important role in hepatitis B virus infection
doi: 10.1371/journal.ppat.1009722
Figure Lengend Snippet: The cell culture supernatant of Huh7 cells, which contained high level of apoE, was mixed with heparin-conjugated agarose beads in the absence or presence of varying amounts (0, 2.5, 5, 10, 20 μg/ml) of a purified human LDLR. Upon washing with PBS, the heparin-bound (pulldown) and unbound apoE protein was determined by Western blot analysis using apoE-specific WuE4 monoclonal antibody ( A ). The percentage of heparin-bound and unbound apoE is plotted using average values obtained from three repeats ( B ).
Article Snippet: ApoE-specific monoclonal antibody WuE4 (ATCC) was produced in the lab as described previously [ ].
Techniques: Cell Culture, Purification, Western Blot